Up-regulation Of EIF3e Is Associated with The Progression of Esophageal Squamous Cell Carcinoma and Poor Prognosis in Patients

Xu, Fengkai; Gu, Jie; Wang, Lin; Liu, Ronghua; Yuan, Yunfeng; Wang, Hao; Jiang, Jiahao; Mao, Wei; Lu, Chunlai; Ge, Di

doi:10.7150/jca.22546

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J Cancer 2018; 9(7):1135-1144. doi:10.7150/jca.22546 This issue Cite

Research Paper

Up-regulation Of EIF3e Is Associated with The Progression of Esophageal Squamous Cell Carcinoma and Poor Prognosis in Patients

Fengkai Xu^1*, Jie Gu^1*, Lin Wang¹, Ronghua Liu², Yunfeng Yuan¹, Hao Wang¹, Jiahao Jiang¹, Wei Mao¹, Chunlai Lu^1,✉, Di Ge^1,✉

1. Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, P. R. China.
2. Department of Immunology and Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences, Fudan University, Shanghai, P. R. China
^*These two authors contributed equally to this work.

Citation:

Xu F, Gu J, Wang L, Liu R, Yuan Y, Wang H, Jiang J, Mao W, Lu C, Ge D. Up-regulation Of EIF3e Is Associated with The Progression of Esophageal Squamous Cell Carcinoma and Poor Prognosis in Patients. J Cancer 2018; 9(7):1135-1144. doi:10.7150/jca.22546. https://www.jcancer.org/v09p1135.htm

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Abstract

Introduction: Esophageal cancer is one of the most common malignant tumors in the world. Eukaryotic translation initiation factors 3e (eIF3e) makes a notable difference in the initiation of protein synthesis and tumor progression. However, the role of eIF3e in ESCC has not been revealed yet. This study aims to investigate the bio-functional and prognostic role of eIF3e in human ESCC tissues and cells.

Methods: Immunohistochemical staining and Western blot were performed to detect the eIF3e expression in ESCC patients' tissues. The Kaplan-Meier product limit method and Cox regression were conducted to analyze the association between eIF3e expression, together with other related clinical/pathological features, and patients' prognosis. In the analysis of bio-functional role of eIF3e, CCK-8 and Transwell assay were performed to compare the proliferative and migrative ability after knockdown of eIF3e.

Results: Up-regulation of eIF3e were demonstrated in ESCC tissues compared with the corresponding para-cancerous tissues. Overexpression of eIF3e was associated with deep tumor depth, lymph nodes metastasis, and advanced TNM stage. Importantly, the patients with high eIF3e expression suffered shorter overall and disease-free survival. Lymph node metastasis and histological grade served as independent prognostic predictors in patients' prognosis. Knockdown of eIF3e could inhibit cell proliferation and migration, in vitro.

Conclusions: The eIF3e expression, or combined with other members of eIF3 complex, might predict poor prognosis of ESCC and serve as a potential breakthrough in the multimodality therapy of ESCC.

Keywords: Esophageal squamous cell carcinoma, Eukaryotic translation initiation factors 3e, prognosis

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APA

Xu, F., Gu, J., Wang, L., Liu, R., Yuan, Y., Wang, H., Jiang, J., Mao, W., Lu, C., Ge, D. (2018). Up-regulation Of EIF3e Is Associated with The Progression of Esophageal Squamous Cell Carcinoma and Poor Prognosis in Patients. Journal of Cancer, 9(7), 1135-1144. https://doi.org/10.7150/jca.22546.

ACS

Xu, F.; Gu, J.; Wang, L.; Liu, R.; Yuan, Y.; Wang, H.; Jiang, J.; Mao, W.; Lu, C.; Ge, D. Up-regulation Of EIF3e Is Associated with The Progression of Esophageal Squamous Cell Carcinoma and Poor Prognosis in Patients. J. Cancer 2018, 9 (7), 1135-1144. DOI: 10.7150/jca.22546.

NLM

CSE

Xu F, Gu J, Wang L, Liu R, Yuan Y, Wang H, Jiang J, Mao W, Lu C, Ge D. 2018. Up-regulation Of EIF3e Is Associated with The Progression of Esophageal Squamous Cell Carcinoma and Poor Prognosis in Patients. J Cancer. 9(7):1135-1144.

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