J Cancer 2017; 8(14):2699-2703. doi:10.7150/jca.19947

Short Research Communication

Evaluation Analysis of miRNAs Overexpression in Liquid-Based Cytology Endometrial Samples

Christine Kottaridi1*✉, Aris Spathis1*, Niki Margari1, Nikolaos Koureas2, Emmanouil Terzakis2, Charalampos Chrelias3, Asimakis Pappas3, Evripidis Bilirakis4,5, Abraham Pouliakis1, Ioannis J Panayiotides6, Petros Karakitsos1†

1. Department of Cytopathology, University General Hospital “ATTIKON”, School of Medicine, National and Kapodistrian University of Athens, Athens 12464, Greece
2. Department of Obstetrics and Gynaecology, “Saint Savvas” Anticancer Hospital, Athens 11522, Greece
3. 3 rd Department of Gynaecology and Obstetrics, University General Hospital “ATTIKON”, School of Medicine, National and Kapodistrian University of Athens, Athens 12464, Greece
4. Department of Obstetrics and Gynaecology, IASO Hospital, 37-39 Kifisias Av., Maroussi, 15123, Athens, Greece
5. Colposcopy Unit, 1 st Department Department of Gynaecology and Obstetrics, General Hospital “Elena Venizelou”, Athens, 11521, Greece
6. 2 nd Department of Pathology, University General Hospital “ATTIKON”, School of Medicine, National and Kapodistrian University of Athens, Athens 12464, Greece
*Joint first authors
In loving memory of a dedicated doctor, an outstanding scientist, and a beloved teacher.

Abstract

Background: miRNAs have an important role as their deregulation is linked to endometrial cancer.

Methods: A custom miScript® miRNA PCR Array was used to investigate for the first time the expression of eight miRNAs in forty-nine histologically confirmed Liquid Based cytology endometrial samples. The expression profile of the same miRNAs was also examined in sixty formalin-fixed tissue samples.

Results: Expression of seven miRNAs was significantly higher in malignant samples with three of them (mir-182, mir-141 and mir-205) performing optimally.

Conclusion: These results suggest the potential use of this non-invasive method of sampling for miRNA expression studies. Furthermore miRNA overexpression could serve as an ancillary or reflex test for optimal identification of malignant samples especially in morphologically inadequate samples.

Keywords: miRNAs, endometrial cancer, Liquid Based Cytology

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How to cite this article:
Kottaridi C, Spathis A, Margari N, Koureas N, Terzakis E, Chrelias C, Pappas A, Bilirakis E, Pouliakis A, Panayiotides IJ, Karakitsos P. Evaluation Analysis of miRNAs Overexpression in Liquid-Based Cytology Endometrial Samples. J Cancer 2017; 8(14):2699-2703. doi:10.7150/jca.19947. Available from http://www.jcancer.org/v08p2699.htm