J Cancer 2017; 8(6):1062-1070. doi:10.7150/jca.17080
The Relation of HPV Infection and Expression of p53 and p16 Proteins in Esophageal Squamous Cells Carcinoma
1. Teaching and Research Institute, Molecular Oncology Research Center, Barretos Cancer Hospital - Pio XII Foundation, Brazil;
2. Department of Endoscopy, Barretos Cancer Hospital - Pio XII Foundation, Brazil;
3. Medical Ambulatory of Specialty Clinics - Barretos, Brazil;
4. Molecular Biology Laboratory, Center for Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo - ICESP, São Paulo, Brazil;
5. Department of Radiology and Oncology, School of Medicine, University of São Paulo, Brazil;
6. Department of Clinical Research - Biohit Oyj, Finland;
7. Medical Laboratory of Medical Investigation (LIM) 14.Department of Pathology, Faculty of Medicine, University of São Paulo, Brazil;
8. Research Institute of Life and Health Sciences (ICVS), University of Minho, Braga, Portugal;
9. ICVS / 3B's - Associated Laboratory to the Government of Portugal, Braga / Guimarães, Portugal.
GOAL: To investigate the HPV prevalence and characterize the expression of potential molecular surrogate markers of HPV infection in esophageal squamous cell carcinoma.
MATERIALS AND METHODS: The prevalence of HPV in individuals with and without esophageal cancer (EC) was determined by using multiplex PCR; p16 and p53 protein levels were assessed by immunohistochemistry (IHC).
RESULTS: High-risk HPV (hr-HPV) was found in the same frequency (13.8%) in esophageal squamous cell carcinoma (ESCC) and in healthy individuals. The p53 expression was positive in 67.5% of tumor tissue, 20.0% of adjacent non-tumoral tissue and 1.8% of normal esophageal tissue. p16 was positive in 11.6% of esophageal cancer cases and 4.7% of adjacent non-tumoral tissue. p16 was undetectable among control group samples. p53 and p16 levels were not significantly associated with the HPV status.
CONCLUSIONS: These results suggest that hr-HPV types are not associated with the development of ESCC and that p53 and p16 protein expression have no relationship with HPV infection in normal or cancerous esophagus.
Keywords: Esophageal neoplasias, p16, p53, immunohistochemistry, HPV.
Pastrez PRA, Mariano VS, da Costa AM, Silva EM, Scapulatempo-Neto C, Guimarães DP, Fava G, Neto SAZ, Nunes EM, Sichero L, Villa LL, Syrjanen KJ, Longatto-Filho A. The Relation of HPV Infection and Expression of p53 and p16 Proteins in Esophageal Squamous Cells Carcinoma. J Cancer 2017; 8(6):1062-1070. doi:10.7150/jca.17080. Available from http://www.jcancer.org/v08p1062.htm