J Cancer 2017; 8(6):1062-1070. doi:10.7150/jca.17080

Research Paper

The Relation of HPV Infection and Expression of p53 and p16 Proteins in Esophageal Squamous Cells Carcinoma

Paula Roberta Aguiar Pastrez1, Vânia Sammartino Mariano1, Allini Mafra da Costa1, Estela Maria Silva1, Cristovam Scapulatempo-Neto1, Denise Peixoto Guimarães1, 2, Gilberto Fava2, Said Abdala Zemi Neto3, Emily Montosa Nunes4, Laura Sichero4, Luisa Lina Villa4, 5, Kari Juhani Syrjanen1, 6, Adhemar Longatto-Filho1, 7, 8, 9✉

1. Teaching and Research Institute, Molecular Oncology Research Center, Barretos Cancer Hospital - Pio XII Foundation, Brazil;
2. Department of Endoscopy, Barretos Cancer Hospital - Pio XII Foundation, Brazil;
3. Medical Ambulatory of Specialty Clinics - Barretos, Brazil;
4. Molecular Biology Laboratory, Center for Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo - ICESP, São Paulo, Brazil;
5. Department of Radiology and Oncology, School of Medicine, University of São Paulo, Brazil;
6. Department of Clinical Research - Biohit Oyj, Finland;
7. Medical Laboratory of Medical Investigation (LIM) 14.Department of Pathology, Faculty of Medicine, University of São Paulo, Brazil;
8. Research Institute of Life and Health Sciences (ICVS), University of Minho, Braga, Portugal;
9. ICVS / 3B's - Associated Laboratory to the Government of Portugal, Braga / Guimarães, Portugal.

Abstract

GOAL: To investigate the HPV prevalence and characterize the expression of potential molecular surrogate markers of HPV infection in esophageal squamous cell carcinoma.

MATERIALS AND METHODS: The prevalence of HPV in individuals with and without esophageal cancer (EC) was determined by using multiplex PCR; p16 and p53 protein levels were assessed by immunohistochemistry (IHC).

RESULTS: High-risk HPV (hr-HPV) was found in the same frequency (13.8%) in esophageal squamous cell carcinoma (ESCC) and in healthy individuals. The p53 expression was positive in 67.5% of tumor tissue, 20.0% of adjacent non-tumoral tissue and 1.8% of normal esophageal tissue. p16 was positive in 11.6% of esophageal cancer cases and 4.7% of adjacent non-tumoral tissue. p16 was undetectable among control group samples. p53 and p16 levels were not significantly associated with the HPV status.

CONCLUSIONS: These results suggest that hr-HPV types are not associated with the development of ESCC and that p53 and p16 protein expression have no relationship with HPV infection in normal or cancerous esophagus.

Keywords: Esophageal neoplasias, p16, p53, immunohistochemistry, HPV.

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How to cite this article:
Pastrez PRA, Mariano VS, da Costa AM, Silva EM, Scapulatempo-Neto C, Guimarães DP, Fava G, Neto SAZ, Nunes EM, Sichero L, Villa LL, Syrjanen KJ, Longatto-Filho A. The Relation of HPV Infection and Expression of p53 and p16 Proteins in Esophageal Squamous Cells Carcinoma. J Cancer 2017; 8(6):1062-1070. doi:10.7150/jca.17080. Available from http://www.jcancer.org/v08p1062.htm