J Cancer 2017; 8(5):816-824. doi:10.7150/jca.17759

Research Paper

B7-H3 Promotes the Migration and Invasion of Human Bladder Cancer Cells via the PI3K/Akt/STAT3 Signaling Pathway

Yuchao Li1, 2, Guoning Guo3, Jie Song2, Zhiping Cai2, Jin Yang1, Zhiwen Chen4, Yun Wang1, Yaqin Huang1, Qiangguo Gao1✉

1. Department of Cell Biology, Third Military Medical University, Chongqing 400038, China;
2. Trainee Brigade, Third Military Medical University, Chongqing 400038, China;
3. Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing 400038, China;
4. Urology Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.

Abstract

Bladder cancer is one of most common malignant cancer. Although previous studies have found abnormal expression of B7-H3 in human bladder cancer tissues, the exact role and molecular mechanism of B7-H3 in bladder cancer remain unknown. In this study, we first detected the expression of B7-H3 in human bladder cancer samples and cell lines, and analyzed its correlations with clinicopathological pathological parameters. Next, siRNAs or overexpression plasmids of B7-H3 were transfected into T24 or 5637 cells, and cell proliferation, apoptosis, migration and invasion were analyzed via CCK-8, colony formation, flow cytometry and transwell assays, protein expression levels were determined by western blotting. The results presented here showed B7-H3 was upregulated in bladder cancer samples compared with normal tissues, and the expression level was correlated with local invasion status. B7-H3 did not affect cell proliferation and apoptosis, but cell migration and invasion were changed through the regulation of matrix metalloproteinase (MMP) 2/9. Knockdown of B7-H3 resulted in decreased activity of the STAT3 and PI3K/Akt pathways, and the Akt served as an upstream regulator of the STAT3. Our results suggest that the overexpression of B7-H3 promotes the migration and invasion of human bladder cancer cells through the PI3K/Akt/STAT3 signaling pathway.

Keywords: B7-H3, migration, invasion, human bladder cancer, PI3K/Akt/STAT3, MMP

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How to cite this article:
Li Y, Guo G, Song J, Cai Z, Yang J, Chen Z, Wang Y, Huang Y, Gao Q. B7-H3 Promotes the Migration and Invasion of Human Bladder Cancer Cells via the PI3K/Akt/STAT3 Signaling Pathway. J Cancer 2017; 8(5):816-824. doi:10.7150/jca.17759. Available from http://www.jcancer.org/v08p0816.htm