J Cancer 2016; 7(8):910-914. doi:10.7150/jca.14714

Research Paper

Effect of Irradiation on Tissue Penetration Depth of Doxorubicin after Pressurized Intra-Peritoneal Aerosol Chemotherapy (PIPAC) in a Novel Ex-Vivo Model

Veria Khosrawipour1,2*, Urs Giger-Pabst1,2*, Tanja Khosrawipour2, Yousef Hedayat Pour3, David Diaz-Carballo4, Eckart Förster5, Hugo Böse-Ribeiro3, Irenäus Anton Adamietz3, Jürgen Zieren1, Khashayar Fakhrian3 ✉

1. Department of General Surgery & Therapy Center for Peritonealcarcinomatosis, Marien Hospital Herne, Ruhr University Bochum, Germany.
2. Basic Research Laboratory, Department of Surgery, Marien Hospital Herne, Ruhr University Bochum, Germany.
3. Department of Radiation Oncology, Marien Hospital Herne, Ruhr University Bochum, Germany.
4. Department of Hematology & Medical Oncology, Marien Hospital Herne, Ruhr University Bochum, Germany.
5. Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Germany.
* Veria Khosrawipour and Urs Giger-Pabst have equally contributed to this study, and both authors should be considered first author.

Abstract

Background: This study was performed to assess the impact of irradiation on the tissue penetration depth of doxorubicin delivered during Pressurized Intra-Peritoneal Aerosol Chemotherapy (PIPAC).

Methods: Fresh post mortem swine peritoneum was cut into 10 proportional sections. Except for 2 control samples, all received irradiation with 1, 2, 7 and 14 Gy, respectively. Four samples received PIPAC 15 minutes after irradiation and 4 other after 24 hours. Doxorubicin was aerosolized in an ex-vivo PIPAC model at 12 mmHg/36°C. In-tissue doxorubicin penetration was measured using fluorescence microscopy on frozen thin sections.

Results: Doxorubicin penetration after PIPAC (15 minutes after irradiation) was 476 ± 74 µm for the control sample, 450 ± 45µm after 1 Gy (p > 0.05), 438 ± 29 µm after 2 Gy (p > 0.05), 396 ± 32 µm after 7 Gy (p = 0.005) and 284 ± 57 after 14 Gy irradiation (p < 0.001). The doxorubicin penetration after PIPAC (24 hours after irradiation) was 428 ± 77 µm for the control sample, 393 ± 41 µm after 1 Gy (p > 0.05), 379 ± 56 µm after 2 Gy (p > 0.05), 352 ± 53 µm after 7 Gy (p = 0.008) and 345 ± 53 after 14 Gy irradiation (p = 0.001).

Conclusions: Higher (fractional) radiation dose might reduce the tissue penetration depth of doxorubicin in our ex-vivo model. However, irradiation with lower (fractional) radiation dose does not affect the tissue penetration negatively. Further studies are warranted to investigate if irradiation can be used safely as chemopotenting agent for patients with peritoneal metastases treated with PIPAC.

Keywords: ex-vivo, penetration, Pressurized Intra-Peritoneal Aerosol Chemotherapy (PIPAC), peritoneal carcinomatosis, radiotherapy.

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How to cite this article:
Khosrawipour V, Giger-Pabst U, Khosrawipour T, Pour YH, Diaz-Carballo D, Förster E, Böse-Ribeiro H, Adamietz IA, Zieren J, Fakhrian K. Effect of Irradiation on Tissue Penetration Depth of Doxorubicin after Pressurized Intra-Peritoneal Aerosol Chemotherapy (PIPAC) in a Novel Ex-Vivo Model. J Cancer 2016; 7(8):910-914. doi:10.7150/jca.14714. Available from http://www.jcancer.org/v07p0910.htm