J Cancer 2016; 7(4):462-466. doi:10.7150/jca.13566

Research Paper

Newcastle Disease Virus Hemagglutinin Neuraminidase as a Potential Cancer Targeting Agent

Ali Baradaran1, Khatijah Yusoff2,3✉, Norazizah Shafee2,3, Raha Abdul Rahim1,3

1. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia;
2. Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia;
3. Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia.

Abstract

The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) with its immunotherapeutic activities and sialic acid binding abilities is a promising cancer adjuvant. The HN was surfaced displayed on Lactococcus lactis and its cancer targeting ability was investigated via attachment to the MDA-MB231 breast cancers. To surface display the HN protein on the bacterial cell wall, HN was fused to N-acetylmuraminidase (AcmA) anchoring motif of L. lactis and expressed in Chinese hamster ovary cells. The expressed recombinant fusion proteins were purified and mixed with a culture of L. lactis and Lactobacillus plantarum. Immunofluorescence assay showed the binding of the recombinant HN-AcmA protein on the surface of the bacterial cells. The bacterial cells carrying the HN-AcmA protein interacted with the MDA-MB231 breast cancer cells. Direct and fluorescent microscopy confirmed that L. lactis and Lb. plantarum surface displaying the recombinant HN were attached to the breast cancer MDA-MB231 cells, providing evidence for the potential ability of HN in targeting to cancer cells.

Keywords: Hemagglutinin-neuraminidase, Cancer targeting, Lactococcus lactis, Surface display.

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How to cite this article:
Baradaran A, Yusoff K, Shafee N, Rahim RA. Newcastle Disease Virus Hemagglutinin Neuraminidase as a Potential Cancer Targeting Agent. J Cancer 2016; 7(4):462-466. doi:10.7150/jca.13566. Available from http://www.jcancer.org/v07p0462.htm