J Cancer 2016; 7(3):241-250. doi:10.7150/jca.12899
Delivery System of CpG Oligodeoxynucleotides through Eliciting an Effective T cell Immune Response against Melanoma in Mice
1. Department of Molecular Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China
2. Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China
3. NSF Nanoscale Science and Engineering Center (NSEC), The Ohio State University, Columbus, OH 43212, USA
Purpose: In order to improve the immunogenicity of whole tumor cell lysate for tumor vaccine, we have designed a series of CpG ODNs to study their transport and to evaluate their anti-tumor activity in B16 melanoma mouse models.
Methods: In this study, we investigated whether C-class CpG ODN (CpG ODN-685) could facilitate tumor cell lysate to induce vigorous anti-tumor activity against tumors in mice both prophylactically and therapeutically.
Results: It was found that the combination of tumor cell lysate and CpG ODN-685 could inhibit the growth of B16 melanoma and prolong the survival of tumor-bearing mice. Moreover CpG ODN-685 with the addition of tumor cell lysate can also cause the generation of tumor specific immune memory by inducing specific cytotoxic T lymphocytes and helper T lymphocytes in mice.
Conclusion: The results suggest that CpG ODN-685 could be developed as an efficient adjuvant for tumor vaccines against melanoma.
Keywords: tumor cell lysate, CpG ODN, melanoma, T lymphocytes
Sun W, Fang M, Chen Y, Yang Z, Xiao Y, Wan M, Wang H, Yu Y, Wang L. Delivery System of CpG Oligodeoxynucleotides through Eliciting an Effective T cell Immune Response against Melanoma in Mice. J Cancer 2016; 7(3):241-250. doi:10.7150/jca.12899. Available from http://www.jcancer.org/v07p0241.htm