J Cancer 2015; 6(8):701-708. doi:10.7150/jca.11785 This issue Cite

Research Paper

Anticancer Effect of a Novel Proteasome Inhibitor, YSY01A, via G2/M Arrest in PC-3M Cells in vitro and in vivo

Mei Zhang1,3, Xia Yuan1, Bo Xu1, Wei Guo1, Fu-Xiang Ran1, Run-Tao Li2, Jing-Rong Cui1✉

1. The State Key Laboratory of Natural and Biomimetic Drugs
2. Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
3. Department of pharmacology, School of Pharmaceutical Sciences, Shihezi University, Xinjiang 832000, China

Citation:
Zhang M, Yuan X, Xu B, Guo W, Ran FX, Li RT, Cui JR. Anticancer Effect of a Novel Proteasome Inhibitor, YSY01A, via G2/M Arrest in PC-3M Cells in vitro and in vivo. J Cancer 2015; 6(8):701-708. doi:10.7150/jca.11785. https://www.jcancer.org/v06p0701.htm
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Abstract

YSY01A is a new tripeptideboronic acid and an analog of PS341. However, YSY01A's antitumor effects and mechanism have not yet been elucidated. This study demonstrates that YSY01A inhibited proteasome activity by combining with the chymotrypsin-like (CT-L) site (β5i/β5), the post-glutamyl peptide hydrolase (PGPH) site (β1i/β1) and the trypsin-like (T-L) site (β2i/β2) in special fluorgonic substrates and proteasome probe tests. We explored the anticancer effect using methyl thiazolyltetrazolium (MTT) or sulforhodamine B (SRB), and PC-3M cells were sensitive to YSY01A among the four cancer cell types tested. The YSY01A antiproliferative effect was stronger than that of PS341. In vivo, YSY01A (1.25, 2.25, and 3.25 mg/kg) inhibited PC-3M cell xenograft tumor growth, and the tumor volume inhibition rate was approximately 40% to 60%. YSY01A arrested PC-3M cells in the G2/M phase of the cell cycle by flow cytometry (FCM). Many proteins related to the cell cycle were analyzed using western blot, and YSY01A was shown to increase p21, p27, cyclinB1, P-cdc2 (tyr15) and wee1 protein expression in both cells and tumor tissue in a concentration-dependent manner. YSY01A, a proteasome inhibitor, exerts anticancer effects on PC-3M cells in vitro and in vivo. The mechanism of the YSY01A-mediated antitumor effect is that the cell cycle is arrested at the G2/M stage. This study suggests that YSY01A may be a novel therapeutic agent for prostate cancer.

Keywords: Anticancer, proteasome inhibitor, tripeptideboronic acid, PC-3M cell, cell cycle


Citation styles

APA
Zhang, M., Yuan, X., Xu, B., Guo, W., Ran, F.X., Li, R.T., Cui, J.R. (2015). Anticancer Effect of a Novel Proteasome Inhibitor, YSY01A, via G2/M Arrest in PC-3M Cells in vitro and in vivo. Journal of Cancer, 6(8), 701-708. https://doi.org/10.7150/jca.11785.

ACS
Zhang, M.; Yuan, X.; Xu, B.; Guo, W.; Ran, F.X.; Li, R.T.; Cui, J.R. Anticancer Effect of a Novel Proteasome Inhibitor, YSY01A, via G2/M Arrest in PC-3M Cells in vitro and in vivo. J. Cancer 2015, 6 (8), 701-708. DOI: 10.7150/jca.11785.

NLM
Zhang M, Yuan X, Xu B, Guo W, Ran FX, Li RT, Cui JR. Anticancer Effect of a Novel Proteasome Inhibitor, YSY01A, via G2/M Arrest in PC-3M Cells in vitro and in vivo. J Cancer 2015; 6(8):701-708. doi:10.7150/jca.11785. https://www.jcancer.org/v06p0701.htm

CSE
Zhang M, Yuan X, Xu B, Guo W, Ran FX, Li RT, Cui JR. 2015. Anticancer Effect of a Novel Proteasome Inhibitor, YSY01A, via G2/M Arrest in PC-3M Cells in vitro and in vivo. J Cancer. 6(8):701-708.

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