J Cancer 2014; 5(7):537-547. doi:10.7150/jca.7797

Research Paper

FGF10: Type III Epithelial Mesenchymal Transition and Invasion in Breast Cancer Cell Lines

Ali Abolhassani1,2*, Gholam Hossein Riazi1*, Ebrahim Azizi2, Saeid Amanpour3, Samad Muhammadnejad3, Mahnaz Haddadi3, Ali Zekri4, Reza Shirkoohi1 ✉

1. Group of Genetics, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences (TUMS), Tehran, Iran
2. Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
3. Group of experimental research in cancer, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences (TUMS), Tehran, Iran
4. Department of Genetics, Faculty of Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran
* The first two authors have contributed equally to this work.

Abstract

Purpose: Fibroblastic growth factor-10 (FGF-10) has an important role in type I epithelial mesenchymal transition (EMT) during the embryonic period of life (gastrulation). Since EMT has a critical role during cancer cells invasion and metastasis (type III) this study sought to investigate the possible role of FGF-10 in type III EMT by monitoring breast cancer cell lines' behavior by FGF-10 regulation.

Methods: MCF-7 and MDA-MB-231 cell lines with different levels of FGF10 expression were treated with FGF-10 recombinant protein and FGF-10 siRNA, respectively.

Results: The cell viability, migration, colony formation and wound healing have a direct relationship with FGF-10 expression, while FGF-10 expression decreased apoptosis. All mesenchymal factors (such as vimentin, N cadherin, snail, slug, TGF-β) increased due to FGF-10 expression with contrary expression of epithelial markers (such as E-cadherin). Moreover, GSK3β phosphorylation (inactivation) increased with FGF-10 expression.

Conclusion: The important role of FGF-10 in type III EMT on cancer cells and initiation of metastasis via various kinds of signaling pathways has been suggested.

Keywords: FGF10, epithelial mesnchymal transition, invasion, breast cancer, metastasis.

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How to cite this article:
Abolhassani A, Riazi GH, Azizi E, Amanpour S, Muhammadnejad S, Haddadi M, Zekri A, Shirkoohi R. FGF10: Type III Epithelial Mesenchymal Transition and Invasion in Breast Cancer Cell Lines. J Cancer 2014; 5(7):537-547. doi:10.7150/jca.7797. Available from http://www.jcancer.org/v05p0537.htm