S100A1 is a Potential Biomarker for Papillary Thyroid Carcinoma Diagnosis and Prognosis

S100 calcium binding protein A1 (S100A1) is an important member of the S100 family and known to express in a variety of cancers. However, the biological functions of S100A1 in thyroid carcinoma have not been thoroughly studied. In this report, bioinformatics analyses and immunohistochemistry assays were applied to assess the expression profile of S100A1 as well as its relationship with the pathological features and prognosis of papillary thyroid carcinoma (PTC). Meanwhile, functions of S100A1 in PTC cells were analyzed with either in vitro or in vivo experiments. S100A1 was significantly up-regulated in PTC tissues compared with adjacent non-cancerous tissues. S100A1 protein expression was significantly associated with tumor size (p=0.0032) or lymph node metastasis (p=0.0331). More importantly, an elevated S100A1 expression was significantly correlated with a worse recurrence-free survival (RFS) (HR=2.26, p=0.042). Further, knockdown of S100A1 dramatically inhibited cell proliferation and migration as well as increased apoptosis of PTC cells. S100A1 knockdown inhibited tumor progression as seen in in vivo experiments. In terms of mechanism, down-regulation of S100A1 induced yes associated protein (YAP) phosphorylation in the cytoplasm and diminished Hippo/YAP pathway activation. Therefore, S100A1 may serve as a novel oncogene and a promising biomarker for PTC diagnosis and prognosis.


Introduction
Diagnosis of thyroid neoplasm is growing fast worldwide [1]. Majority of the new cases has been attributed to an increase in papillary thyroid carcinoma (PTC) [2,3], which usually has a good prognosis. Although the etiology of PTC remains unclear, increased use of diagnostic imaging has been identified as one of the major driving forces [4,5]. The increased use of diagnostic imaging leads to an over-diagnosis of PTC, which may put patients at risk for over-treatment without conferring a survival benefit [6]. In order to promote individualized treatments, new biomarkers are urgently needed to distinguish PTC patients who need from those who do not need aggressive treatments.
The S100 calcium binding protein family has more than 20 members. By functioning both as extracellular signaling factors and intracellular Ca 2+ sensors [7], they are involved in a variety of cellular biological processes, including cell proliferation, transformation, invasion, and migration [8,9]. Multiple S100 protein family members, like S100A4 [10][11][12], S100A8 [13], S100A9 [14], S100A12 [15], S100A13 [16], have been reported to be involved in the initiation and prognosis of thyroid neoplasms, which suggests a close and complex relationship between S100 proteins and PTC.

Ivyspring
International Publisher and S100A13 [16] play crucial roles in thyroid cancer development and progression, thus can be potential biological markers or therapeutic targets of PTC. It was reported that S100A4 overexpression is a poor prognostic biomarker for thyroid cancer [12]. S100A4 knockout significantly inhibits the survival and invasion of anaplastic thyroid cancer cells and potentiates the effect of vemurafenib on tumor regression [10,19]. Overexpression of S100A4 is also associated with papillary thyroid carcinoma invasion and metastasis. Therefore, S100A4may be a potential target for PTC therapy [11,20]. Further, S100A12 expression is strikingly increased in PTC and strongly associated with TNM stage, tumor size, and lymph node metastasis. Silencing S100A12 reduced the aggressive growth of tumor cells with either in vitro or in vivo experiments [15]. S100A1, also called S100 alpha, is the first discovered member of the S100 family. Previously studies showed that S100A1 could be detected in many tissues. For example, S100A1 is expressed in both heart and skeletal muscle. S100A1 silencing reduces contractility of cardiomyocytes. By contrast, enhanced cardiac S100A1 expression increases energy production and contractility [21,22] and improves recovery of the heart from ischemia-reperfusion injuries [23,24]. S100A1 is also highly expressed in various cancer cells and participates in regulating important pathophysiological processes. For example, expression level of S100A1 is markedly increased in melanoma tissues compared with normal skin tissues [25]. S100A1 is abnormally expressed and serves as an independent prognostic indicator for relapse-free survival in endometrioid subtypes of ovarian carcinoma [26,27]. However, the relationship between S100A1 and PTC is still unclear. In this study, S100A1 expression in human PTC was investigated to determine its diagnostic utility in thyroid cancers. Prognostic value of the biomarker in PTC patients was also evaluated. Meanwhile, its oncogene role was analyzed further with in vitro or in vivo experiments.

Immunohistochemistry
Tissue samples were fixed in paraformaldehyde and embedded in paraffin. After conventional sectioning, de-waxing, and hydrating steps, tissues received antigen repair using citric acid buffer. Endogenous peroxidase was blocked using 3% hydrogen peroxide for 30 minutes. The tissue sections were treated with S100A1 primary antibody (Novus, NBP2-29403, 1:400) overnight at 4℃ then incubated with horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (servicebio, G1216-200T, 1:200) for one hour at room temperature, and then stained with 3,3′-diaminobenzidine (DAB) (servicebio, G1216-200T). Hematoxylin was used to stain nucleus. All immunohistochemical procedure was conducted under the same conditions. Scoring of IHC was performed using the method described by Zhang et al [28]. Briefly, both percentage and staining intensity of tumor cells were scored separately. Score 0 (percentage of positive cells ＜ 5%); Score 1 (percentage of positive cells 5%-25%); Score 2 (percentage of positive cells 26%-50%); Score 3 (percentage of positive cells 51%-75%); Score 4 (percentage of positive cells＞75%). Scale of 0-3 was used for grading staining intensity. Multiplication of the two scores was regarded as the final staining score. For statistical analyses, patients who had a negative or weak S100A1 expression were assigned to the low expression group (score ≤ 4). By contrast, patients with a strong S100A1 expression were assigned to the high expression group (score>4).

Cell culture
Human PTC cell lines K1, TPC1, BCPAP were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). The normal thyroid follicular epithelium cell line (Nthy-ori3-1) was purchased from Chinese Academy of Sciences (Shanghai, China). Short tandem repeat (STR) and amelogenin of all these cell lines had been identified by a third party (Shanghai BioWing). K1, TPC1 and Nthy-ori3-1 were cultured in 10% (v/v) fetal bovine serum (FBS)-supplemented 1640 medium (Gibco, Grand Island, USA). BCPAP cells were cultured in 1640 medium with 1× Non-Essential Amino Acids Solution (NEAA; Life Technologies) and 10% FBS. Cells were cultured in the incubator at 37 degree Celsius (°C) containing 5% CO2. Culture medium was changed every other day, and cells were passaged when the density reached about 80% confluence.
Cell transfection S100A1-RNAi siRNA were purchased from Guangzhou ribobio Company (Guangzhou, China). The target sequences for S100A1-Si1, S100A1-Si2 and S100A1-Si3 were GGAGCTAGACGAGAATGGA, CCAGGAGTATGTGGTGCTT, ACAGTGGCCTGTAACAATT respectively. Thyroid cancer cells were transfected according to the manufacturer's protocol. Silencing efficiency was evaluated by qRT-PCR and Western blot (48 and 72 hours after transfection respectively). The S100A1 lentivirus and negative control lentivirus were purchased from Genechem company (Shanghai, China). The S100A1-shRNA1 target sequence was GGAGCTAGACGAGAATGGA, the S100A1-shRNA2 target sequence was ACAGTGGCCTGTAACAATT, and the negative control shRNA was TTCTCCGAACGTGTCACGT.
These DNA oligonucleotides were inserted into hU6-MCS-Ubiquitin-EGFP-IRES-puro lentiviral vector. Transfection efficiency of lentivirus was assessed with qRT-PCR and Western blot assays.

Cell Counting Kit-8 and Colony formation assays
Cell growth rate was assessed by Cell Counting Kit-8 (CCK-8) assays. 3,000 cells were seeded per well in a 96-well plate at 0 hour, and the absorbance was measured every 24 hours for 4 days. Before testing started, the medium was changed to 1640 medium containing 10% CCK-8 (100ul/well, Dojindo, Japan). After incubated for one hour at 37°C, the optical density (OD) value was measured using the microplate reader at the wavelength of 450nm. Colony formation assays were conducted to detect the ability of a single cell to grow into a colony. PTC cells were seeded in a six-well plate at the same density (2,000 cells/well). After cultured for 14 days, the clones were fixed by 4% paraformaldehyde and stained with 0.1% crystal violet. Visible PTC cell clones were counted using the ImageJ software.

Cell migration assays
The migration capacity of cells was evaluated by using the 24-well transwell chamber with 8μm pore sizes (Corning, USA). Cells (2×10 4 cells/well) were seeded in the upper chambers in serum-free medium, and the lower chambers were filled with 1640 medium containing 15% FBS as a chemo-attractant. After being cultured in a 37°C incubator for 12 hours, PTC cells in the upper chambers were carefully cleaned, while invaded PTC cells at the lower membrane surface were fixed with 4% paraformaldehyde and stained with crystal violet. Finally, those cells were sealed with neutral resin and observed under a microscope. Three visual fields in each sample were randomly selected to count the invaded cells.

Flow cytometric analysis
Apoptosis was measured by Annexin V/PI staining using the Apoptosis Detection Kit (BD Biosciences). PTC cells were stained with Annexin V-FITC and PI by following the instructions, and then examined by means of the FACScan flow cytometer. The percentage of apoptotic cells was calculated as the sum of the early and late apoptosis rates. For cell cycle experiments, cells were washed with cold PBS and fixed in 85% ethanol at -20℃ overnight. RNase (8 μg/ml, AntGene) and propidium iodide (50 μg/ml, Sigma Aldrich) were used to treat cells at 37 °C for 30 minutes. FACScan flow cytometer was used for analyzing the cell cycle distribution.

Nude mouse xenograft model
All mouse care and operations were carried out in accordance with the Tongji Medical University Institutional Animal Care and Use Committee regulations. Five-week-old female BALB/c nude mice were purchased from Charles River Laboratories (Beijing China). 100 ul sterile PBS containing 1×10 7 cells mixed with 100 ul Matrigel Matrix (356234, Corning), which was carefully injected subcutaneously into the right armpit of each nude mouse. Tumor dimensions were monitored every three days to measure tumor sizes utilizing the following formula: volume=1/2(length×width 2 ). 24 days later, all the nude mice were sacrificed. Those tumor tissues were removed and subjected to H&E staining and IHC studies.

TCGA data acquisition and analysis
The raw RNA sequencing data and corresponding clinical information of thyroid carcinoma patients was obtained from The Cancer Genome Atlas (TCGA) database. S100A1 mRNA expression data were extracted as well. For all subsequent analyses, original data were log2 transformed for normalization, and then the difference between cancer and thyroid was estimated. We also analyzed multiple tissue subtypes of PTC to analyze whether there was a relationship between the expression of S100A1 and PTC subtypes. In addition, differential CpG islands methylation expression in the promoter region of the S100A1 gene was analyzed by querying the UALCAN portal. Relative methylated levels of thyroid cancer and normal thyroid tissues were presented in units of beta values, ranging from 0 (no methylation) to 1 (full methylation).

Survival analysis of S100A1
The prognostic values of S100A1 in patients with thyroid cancer were evaluated by Kaplan-Meier plotter (KM-plotter) online database. According to the median expression value of S100A1, all thyroid cancer patients were divided into high-expression and low-expression groups, and the recurrence-free survival (RFS) rate was calculated. The hazard ratio (HR) and 95% confidence interval (CI) were calculated and reported, and the curves were statistically compared using the log-rank test. We followed up 86 PTC patients until November 2020 via telephone calls. Patients were divided into S100A1 high and low expression groups according to median S100A1 expression level. Kaplan-Meier survival analysis was performed and expressed as survival rate in percentage.

Statistical analysis
Statistical analyses were performed using the SPSS (version 20; SPSS Inc. USA), and graphs were made with the GraphPad Prism (version 8.00; GraphPad Software Inc.). Receiver operating characteristic (ROC) curves, area under the curve (AUC), the sensitivity and specificity were also performed using GraphPad Prism 8. All experiments were performed independently for at least three times. Means of two matched/unmatched groups were compared using the paired/unpaired T tests. One-way ANOVA or two-way ANOVA were utilized for comparing the means of three or more comparison groups. P< 0.05 was used to indicate a statistically significant difference.

S100A1 is up-regulated in PTC tissues and correlated with pathological features
Correlation between S100A1 protein and pathological features were investigated using tissue samples from PTC patients. IHC showed S100A1 staining was positive both in the nucleus and cytoplasm of PTC cells, while the S100A1 staining was negative or weak in the normal adjacent tissues (Fig.  1A). S100A1 protein showed a higher expression in PTC tissues than in normal thyroid tissues (Fig. 1B), which is in agreement with the bioinformatics analysis results (Fig. 1C, 1D). No any other histological subtype of PTC was found in the specimens we collected. Thus, only classic PTC was studied in our research. We analyzed the expression of S100A1 in normal thyroid tissue and different histological subtype of PTC (classical PTC, tall cell PTC, follicular PTC) in TCGA database. As shown in Figure 1E, compared with normal thyroid tissue, the expression of S100A1 in various PTC subtypes was higher and the highest in the tall cell PTC. Methylation level of S100A1 gene promoter in thyroid cancer was lower than in normal thyroid tissue, which suggested difference in gene expression might due to different methylation levels (Fig. 1F). Kaplan-Meier survival curve analysis showed that thyroid cancer patients expressing higher S100A1 had a less favorable RFS (Fig. 1G). The telephone follow-up data for all 86 patients were as following: 10 patients could not be contacted, 75 patients were alive and recovered well after the surgery, and one patient died of heart disease. No evidence of local recurrence or distant metastasis was observed in these patients. These follow-up data were expected, probably due to the slow progression and indolent nature of the disease. We performed a survival analysis for DFS based on these data, and found no statistical difference between the S100A1 high and low group (Fig. 1H). This is inconsistent with the results of the previous TCGA data analysis (Fig. 1G), which may be due to the small sample size and a shorter follow-up. Associations between S100A1 protein expression levels and pathological features were summarized in Table 1. High expression of the S100A1 was associated with tumor size (p=0.0032), lymph node metastasis (p=0.0331), but not with gender, age, extrathyroidal extension or bilateral PTC. All these results indicated that S100A1 protein was up-regulated in PTC compared with matched normal thyroid tissues. Patients with high S100A1 expression were more likely to have more aggressive pathological features and a worse prognosis. Statistical significance was tested using Chi-square analysis (* P＜0.05).

S100A1 as a molecular biomarker for PTC diagnosis
ROC curves revealed that S100A1 exhibited an excellent diagnostic efficiency for PTC (AUC=0.8873, p<0.0001; Fig. 1I). The optimal cut-point by Youden index for the predicted threshold of S100A1 was an expression value of 0.719 for PTC (Youden index: 0.773; Sensitivity: 82.46%, Specificity: 94.83%). Moreover, the diagnostic efficiency of S100A1 had high quality among all clinical stages (stage I: AUC=0.8772, stage II: AUC=0.8401, stage III: AUC=0.8945, stage IV: AUC=0.9680; p<0.0001 for all), especially in stage IV, suggesting S100A1 as a fine molecular biomarker for PTC diagnosis.

SiRNA transfection downregulates S100A1 protein in PTC cell lines
In order to further explore the expression of S100A1 in thyroid and papillary thyroid carcinoma in humans, we collected 4 PTC tissues and paired adjacent normal thyroid tissues, and compared the expression of S100A1 protein by Western blot assays ( Fig. 2A). Consistent with the results of our previous immunohistochemical experiments, the expression of S100A1 in PTC was significantly higher than in normal thyroid. Basal expression of S100A1 in the normal thyroid follicular epithelium cell line (Nthy-ori3-1) and three PTC cell lines (BCPAP, TPC-1, K1) were examined by qRT-PCR and Western blot assays. TPC-1 cell line showed the lowest level, while BCPAP, K1 and Nthy-ori3-1 showed high levels of S100A1 expression (Fig. 2B, 2C). Expression of S100A1 in Nthy-ori3-1 cell line was abnormally increased, which may be due to cell line dedifferentiation [29,30]. To validate the functions of S100A1 in PTC cells, we selected K1 and BCPAP, the two PTC cell lines with high S100A1 expression, for carrying out further experiments. S100A1 siRNA knockdown experiments were conducted to test its functions. Transfection efficiency was assessed via qRT-PCR and Western blot assays (Fig. 2D, 2E). S100A1 expression was greatly decreased in siRNA groups compared to either blank or negative control groups (especially for the Si-1 groups), which indicated that S100A1 expressions were successfully knocked down.

S100A1 regulates the tumorigenicity of PTC cells in vitro
CCK8 and colony formation assays were carried out in order to ascertain whether S100A1 affects cell proliferation. Cell growth curves indicated that down regulation of S100A1 significantly inhibited the proliferation of K1 and BCPAP cells when compared to control groups (Fig. 2F and 2G). Consistent with the CCK8 results, colony formation assays revealed that colonogenic ability was also suppressed when S100A1 was knocked down (Fig. 2H and 2I). Transwell assays were performed to see whether S100A1 also regulated cell migration. As shown in Fig. 3A and 3B, the number of cells that migrated into the lower surface in the Si-1 groups were less than the counterpart measure in the negative control (NC) groups for either K1 or BCPAP cell line. Taken together, these results indicated that proliferation and migration of PTC cells were inhibited by the S100A1 knockdown.

S100A1 knockdown induces apoptosis of PTC cells
Many factors including cell senescence and apoptosis could influence cell proliferation. So S100A1 was studied for its possible effects on cell cycle and apoptosis. The percentages of apoptotic cells were significantly increased in Si-1 groups compared to the NC groups (Fig. 3C and 3D). However, the proportion of cells in various phases of the cell cycle was not significantly different between the experimental groups and control groups (data not shown).

S100A1 regulates the tumorigenicity of PTC cells
To further delineate the role of S100A1 in PTC, we performed in vivo experiments with nude mouse xenograft models using K1 cells. First, K1 cells with stably silenced S100A1 were constructed via lentivirus. qRT-PCR and Western blot assays were used to ensure the transfection efficiency after S100A1 specific shRNA transfection (Fig. 4A). Compared with the NC group (sh-NC), sh1 and sh2 groups both had relatively lower mRNA and protein expression, and sh1 group had the lowest expression. Then, K1-sh1 and K1-shNC cells were chosen for performing animal experiments. The volume of tumors in sh1 group was measured 24 days after subcutaneous injection, which were significantly smaller than the counterpart measure in the sh-NC group (Fig. 4B). Consistent with previous results, IHC data also showed the S100A1 expression in the sh1 group was lower than the sh-NC group (Fig. 4C).

Downregulation of S100A1 suppresses PTC cell growth through Hippo/YAP signaling
Many studies [31][32][33] have illustrated that Hippo/YAP pathway played an important role in tissue homoeostasis. Therefore, we investigated whether there was a relationship between S100A1 and Hippo/YAP signaling pathway. Through transient transfection in K1 and BCPAP cell lines, we found that the down regulation of S100A1 was always accompanied by a decrease of nuclear and cytoplasmic YAP protein. By contrast, knockdown of S100A1 increased the phosphorylated YAP in cytoplasm (Fig. 4D). To sum up, our data suggested S100A1 might regulate the Hippo signaling pathway to promote PTC cells proliferation and suppress apoptosis (Fig. 5).  A diagram of the potential underlying regulatory mechanism of S100A1. After phosphorylation and ubiquitination, YAP is degraded by proteasomes in cell cytoplasm. S100A1 could suppress this process, resulting in YAP protein accumulation in cytoplasm and an increase in YAP entering the nucleus, and ultimately induces the transcription of antiapoptotic and proliferative genes. YAP: yes associated protein.

Discussion
The increased incidence of thyroid cancer is mainly attributed to detection of small PTC, which has a generally good prognosis [34]. Of interest, with the similar histopathological features, PTC exhibited varied clinical features and prognoses. Still, about 10% of PTC patients develop metastasis and lead to fatal outcomes [35]. Therefore, novel biomarkers for PTC diagnosis and prognostic assessment are urgently needed. S100A1 is a member of the S100 calcium binding proteins family. Similar to most S100 family proteins, conformational changes take place in S100A1 upon binding calcium and interacting with many target proteins [8]. Through specific interactions, S100A1 is involved in many pathophysiological processes such as synapse release [36], cytoskeletal proteins dynamics [32], and regulation of transcription factors [37]. S100A1 was also reported to be involved in the occurrence and progression of tumors. For example, S100A1 is a novel biomarker in differentiating prostatic adenocarcinoma from nephrogenic adenoma [38]. S100A1 was also suggested to be an immunohistochemical biomarker to distinguish chromophobe renal cell carcinoma from benign renal oncocytoma [39,40]. Li et al. found that S100A1 was expressed significantly higher in melanoma samples than in normal skin samples, which is also associated with clinical pathology such as lymphatic and distant metastasis [25].
A simplified flowchart illustrating the various experimental steps of the study was shown in Fig. 6. In brief, we first analyzed the differential expression of S100A1 in human PTC and normal thyroid tissue. IHC and Western blot assay findings showed that S100A1 protein was remarkably up-regulated in PTC samples and associated with corresponding pathological changes. Additionally, a series of cellular functional experiments were performed to explore the role of S100A1 in PTC, such as CCK-8 assays, colony formation assays, cell migration assays and flow cytometric analysis. Animal experiments also demonstrated that knockdown of S100A1 inhibited PTC development. Finally, the molecular mechanisms behind these differences were explored. Figure 6. A simplified flowchart illustrating the various experimental steps of the study. Immunohistochemistry and Western blot assay findings showed that S100A1 protein was up-regulated in PTC samples. The role of S100A1 in PTC tumorigenesis was investigated by Cell Counting Kit-8 and colony formation assays, flow cytometric analysis, cell migration assays, and in vivo experiments.
The discrepancy on S100A1 protein expression may be due to different tissue types. Our IHC and Western blot assay data showed that S100A1 protein had markedly increased expression in PTC tissues than normal thyroid tissues, which suggests S100A1 may act as an oncogene in PTC. In addition, the ROC curves suggested that a high S100A1 expression was able to distinguish PTC from healthy subjects among all clinical stages, for serving as a robust diagnostic biomarker for PTC. Survival curve analysis based on TCGA dataset showed that the long-term prognosis was quite different between the S100A1 high and low groups, which suggested S100A1 can be used as a prognostic marker for cancer. However, our own survival data did not match TCGA dataset analysis, which may be due to limitations of a small sample size and a shorter follow-up. We will continue to monitor the disease progression of each patient in the studied cohort.
The functions of S100A1 in PTC cell lines were further shown with CCK-8 and colony formation assays, which indicated that S100A1 knockdown inhibited PTC cells proliferation in vitro. Moreover, the transwell assays demonstrated that PTC cells migration was inhibited by S100A1 silencing. Knocking down S100A1 inhibited the progression of PTC cells. Abnormal cell cycle and cell apoptosis are causes of tumorigenesis and cancer progression.
Experiments were designed to investigate the reasons for a slow proliferation, in which S100A1 knockdown induced apoptosis but did not have a significant effect on cell cycle.
To confirm our findings with in vivo experiments, we analyzed the impact of S100A1 silencing in xenograft tumor nude mouse models. Tumors with S100A1 knockdown were comparatively small, which indicated that cell proliferation was greatly inhibited by S100A1 shRNA treatment compared to the negative control. Knockdown of S100A1 could suppress PTC cells in vivo, which suggests S100A1 may act as a novel oncogene in the development and progression of PTC.
To further explore the potential signaling pathway underlying PTC progression, we investigated the association between S100A1 and Hippo signal pathway through measuring expressions of phosphorylated YAP and total YAP. The Hippo/YAP signaling pathway plays a major role in cell proliferation and tissue homoeostasis. The pathway is a protein kinase cascade. With receiving an activating signal, the mammalian Ste20-like kinases 1/2 (MST1/2) is phosphorylated, which activates large tumor suppressor (LATS1/2), and then inhibits Yes-associated protein (YAP) phosphorylation. It was reported S100A1 inactivated the Hippo signaling pathway in hepatocellular carcinoma [41]. Specifically, down-regulation of S100A1 reduced total and nuclear YAP protein but induced YAP phosphorylation in the cytoplasm. Down-regulation of S100A1 upregulated the phosphorylation of LATS1 but did not change the expressions of MST1/2 or p -MST1/2 [41]. Additionally, S100A1 protein could promote chondrogenesis and chondrocyte phenotype maintenance by inhibiting Hippo/YAP pathway via LATS kinases and IQGAP1 (IQ motif containing GTPase activating protein 1) [32,42]. Our findings are consistent with previous reports. Huang et al. found that YAP expression was significantly correlated with the TNM stage, lymph node metastasis, and inferior recurrence-free survival in patients with PTC [43]. YAP also plays an important role in PTC cellular proliferation and anti-autophagy [44,45].

Conclusion
In summary, our findings strongly support that S100A1 promotes papillary thyroid carcinoma cells proliferation and migration through regulating the Hippo/YAP signaling pathway, and functions as a novel biomarker for diagnosis and prognosis of papillary thyroid carcinoma. Inhibitor of S100A1 could also provide a new approach for treating papillary thyroid cancer.